【关 键 词】《中国实验血液学杂志》 hepcidin 【摘 要】1、蛋白名称都按照国际文献常用的拼写法,读者一看就懂。名称中的英文字母一般采用英文大写正体字母拼写,例如BCL-2蛋白,不要写成bcl-2或Bcl-2蛋白;又例如TfR2蛋白(转铁蛋白受体2),按国际文献的惯例,其名称拼写中兼有大小写字母。2、基因名称是其蛋白产物名称的全部字符都改为斜体,原蛋白名称中的英文字母大小写不变而改为斜体,名称中的数字符号也一律改为斜体。例如BCL-2,TfR2。蛋白名称中如有希文字母,改为基因名称时希文字母大小写不变,只把所有字符都 Hepcidin对人非小细胞肺癌H520细胞生长的影响研究^①[J].中国免疫学杂志,2019,第15期 目的:探讨Hepcidin表达对肺癌细胞活力、凋亡率的影响。方法:将人非小细胞肺癌H520细胞分为对照组、si-Scrabble组和si-Hepcidin组,Western blot检测转染siRNA后Hepcidin和Ferroportin蛋白表达;CCK8法检测细胞活力;流式细胞术检测细胞凋亡率;免疫荧光法测定各组细胞胞内铁含量。将转染si-Hepcidin的H520细胞分为Hepcidin~+组和Hepcidin~-组,CCK8法及流式细胞术分别检测细胞活力及凋亡率。结果:转染si-Hepcidin的H520细胞Hepcidin蛋白表达明显低于对照组(P<0.05)。与对照组比较,si-Hepcidin组细胞活力明显降低,凋亡率明显升高,Ferroportin表达明显升高,胞内铁含量明显降低(P<0.05)。Hepcidin~+组细胞活力明显高于Hepcidin~-组,凋亡率低于Hepcidin~-组(P<0.05)。结论:高表达Hepcidin可促进肺癌细胞生长,抑制Hepcidin表达可通过调控肺癌细胞铁代谢抑制细胞活力和诱导细胞凋亡。 益气维血胶囊联合人重组促红素治疗肿瘤相关性贫血疗效及对患者Hepcidin、TFR2水平影响^*[J].陕西中医,2019,第12期 目的:探究益气维血胶囊联合人重组促红素（EPO）对肿瘤相关性贫血患者铁调素（Hepcidin）和转铁蛋白受体2（TFR2）水平的影响及其临床效果研究。方法:选取符合标准的112例患者纳入研究,随机分为对照组和研究组,每组56例。对照组使用EPO治疗,研究组在对照组基础上联合益气维血胶囊治疗。对照两组临床疗效、Hepcidin和TFR2水平。结果:治疗后两组红细胞计数（RBC）、血红蛋白（Hb）和平均血红蛋白浓度（MCHC）均明显升高,研究组各指标分别为（4.27±0.74）×10¹²/L、（91.25±10.02）g/L和（314.5±13.76）g/L,其变化幅度较对照组更大且更趋于正常值（P0.05）。结论:益气维血胶囊联合EPO治疗肿瘤相关性贫血疗效显著,可有效改善Hepcidin和TFR2水平,同时不增加副反应发生率,安全性较好。 鱼类抗菌肽研究进展（二）[J].水产养殖,2019,第11期 3 Hepcidin Hepcidin是一种富含半胱氨酸的多肽,最先在人源的hepcidin中发现其具有抗菌活性。此后,hepcidin在许多其他的脊椎动物被陆续发现,包括爬行类,两栖类和鱼类。在鱼类中,hepcidin最先从杂交条纹鲈中发现,目前至少已经在37种鱼类中鉴定到了该抗菌肽[1]。Hepcidin一般是一个由4个二硫桥形成harpin-折叠,包含8个半胱氨酸,然而鱼hepcidin序列分析表明,鱼类hepcidin只含有4,6或7个半胱氨酸[2]。鱼类hepcidin基因在进化过程中发生了重复和多样化的过程中,产生了多重基因拷贝的,最多 鱼类抗菌肽研究进展（一）[J].水产养殖,2019,第9期 抗菌肽是一类广泛存在于自然界中的高度保守的多肽,在体外和体内都表现出广谱抗菌活性。抗菌肽最早在两栖动物中发现,随后在兔和人类中发现^[1]。鱼类中发现的最早的抗菌肽是1980年从美洲鲽中发现的,但直到1996年后才有突破性的进展,目前鱼类抗菌肽研究已取得与其他脊椎动物物种相同的进展,发现了piscidin家族(鱼类特有的抗 COPD合并呼吸衰竭患者血清HEPCIDIN表达及与预后评估关系[J].河北医学,2019,第9期 目的:探讨COPD合并呼吸衰竭患者血清HEPCIDIN表达及与预后评估。方法:本院COPD合并呼吸衰竭患者115例为病例组,我院门诊健康体检者52例作为对照组,测定各组CRP、WBC、HEPCIDIN、FEV1%、FEV1/FVC、DLCO%水平,同时测定不同程度低氧血症COPD合并呼吸衰竭患者的HEPCIDIN水平。结果:病例组CRP、WBC、HEPCIDIN、IL-6水平高于对照组(P<0.05);病例组HbA1c、FEV1%、FEV1/FVC、DLCO%均低于对照组(P<0.05);随着低氧血症严重程度的增加,HEPCIDIN水平逐渐增加(P<0.01);HEPCIDIN与HbA1c、FEV1%、FEV1/FVC、DLCO%负相关关系明显,与CRP、WBC、IL-6正相关关系明显(P<0.05);高水平的HEPCIDIN、IL-6以及低水平的HbA1c、FEV1/FVC均为COPD合并呼吸衰竭发生死亡事件的危险因素(P<0.05);HEPCIDIN、HbA1c两者的AUC相近,皆小于FEV1/FVC;HEPCIDIN、HbA1c诊断COPD合并呼吸衰竭的灵敏度、特异度相近(χ~2=1.219、1.695,P>0.05),皆小于FEV1/FVC(χ~2=16.249、17.023,P<0.05)。结论:COPD合并呼吸衰竭患者血清HEPCIDIN水平升高,且与COPD疾病严重程度密切相关;高水平的HEPCIDIN、IL-6以及低水平的HbA1c、FEV1/FVC均为COPD合并呼吸衰竭发生死亡事件的危险因素;HEPCIDIN诊断COPD合并呼吸衰竭具有较高的灵敏度、特异度,值得在临床上推广应用。 可手术胃癌和结直肠癌中血清TK1和Hepcidin表达及其与临床病理特征的关系[J].实用癌症杂志,2019,第3期 目的 研究可手术胃癌、结直肠癌患者血清TK1和肿瘤组织Hepcidin表达及其与临床病理特征的关系。方法 应用化学发光方法,检测可手术66例胃癌、结直肠癌患者术前血清TK1表达情况,应用免疫组化方法检测肿瘤组织中Hepcidin表达,并分析TK1与其临床特征的关系。结果 ①66例患者术前血清TK1阳性6例(阳性率9. 09%)。②术前血清TK1表达与患者性别、分化程度、Her-2表达和Ki-67表达有关。但与年龄、T分期、淋巴结转移和贫血均无关(P均> 0. 05)。③Hepcidin在肿瘤组织中阳性表达率为62. 1%。④Hepcidin表达与患者年龄、T分期、Ki-67和脉管浸润有关,而与性别、有无淋巴结转移、分化程度、Her-2表达和有无贫血均无统计学意义(P均> 0. 05)。⑤肿瘤组织Hepcidin表达与血清TK1水平无相关关系(γ=-0. 1005,P> 0. 05)。结论 胃肠道肿瘤组织Hepcidin表达与患者年龄、T分期和脉管浸润和Ki-67有关;术前血清TK1的表达与性别、Her-2表达,分化程度和Ki-67表达有关。联合检测肿瘤组织Hepcidin和术前血清TK1对于指导合理治疗和判断患者的预后有一定意义。 Hepcidin-orchestrated Hemogram and Iron Homeostatic Patterns in Two Models of Subchronic Hepatic injury[J].Biomedical and Environmental Sciences,2019,第3期 Objective This study was designed to evaluate hematological disorders and the orchestrating roles of hepcidin and IL-6 in rat models of thioacetamide（TAA） and carbon tetrachloride（CCl4） hepatotoxicity. Methods Rats were intraperitoneally injected with TAA（10 mg/100 g rat weight dissolved in isosaline） or CCl4（100 μL/100 g rat weight diluted as 1:4 in corn oil） twice weekly for eight consecutive weeks to induce subchronic liver fibrosis. Blood and tissue samples were collected and analyzed. Results CCl4 but not TAA significantly decreased the RBCs, Hb, PCV, and MCV values with minimal alterations in other erythrocytic indices. Both hepatotoxins showed leukocytosis, granulocytosis, and thrombocytopenia. By the end of the experiment, the erythropoietin level increased in the CCl4 model. The serum iron, UIBC, TIBC, transferrin saturation%, and serum transferrin concentration values significantly decreased, whereas that of ferritin increased in the CCl4 model. TAA increased the iron parameters toward iron overload. RT-PCR analysis revealed increased expression of hepatic hepcidin and IL-6 m RNAs in the CCl4 model and suppressed hepcidin expression without significant effect on IL-6 in the TAA model. Conclusion These data suggest differences driven by hepcidin and IL-6 expression between CCl4 and TAA liver fibrosis models and are of clinical importance for diagnosis and therapeutics of liver diseases. 当归多糖对肝细胞Hepcidin表达的影响[J].中国老年学杂志,2018,第19期 目的探讨当归多糖(ASP)对人肝癌细胞Hep G2和人正常肝细胞L-02 Hepcidin表达的抑制作用。方法体外培养Hep G2和L-02细胞,RT-PCR检测不同浓度(0、100、200、400 mg/L)的ASP诱导不同时间(0、24、48、72 h和96 h)对两种细胞Hepcidin mRNA影响。Western印迹观察400 mg/L ASP诱导96 h对两种细胞Hepcidin表达的抑制作用。结果与对照组(0 mg/L)相比,浓度高于200 mg/L ASP能够显著下调Hep G2和L-02细胞Hepcidin mRNA表达(P<0. 001); 400 mg/L ASP连续作用48 h后对Hep G2和L-02细胞产生显著的Hepcidin抑制效应且抑制强度呈时间相关性; 400 mg/L ASP连续作用96 h后显著抑制Hep G2和L-02细胞Hepcidin表达(P<0. 001)。结论 ASP可以抑制Hep G2和L-02细胞中Hepcidin的表达。 Correlation of hepcidin content with iron metabolism, inflammatory response and immune response in patients with rheumatoid arthritis combined with anemia[J].Journal of Hainan Medical College,2018,第16期 Objective:To investigate the correlation of hepcidin content with iron metabolism, inflammatory response and immune response in patients with rheumatoid arthritis combined with anemia.Methods: A total of 50 patients with rheumatoid arthritis combined with anemia who were treated in our hospital between September 2014 and December 2017 were selected as as the study group and 48 patients with rheumatoid arthritis alone who were treated in our hospital during the same period were selected as the control group. The differences in serum levels of hepcidin (Hepc), iron metabolism indexes, inflammatory factors and immune response indexes were compared between the two groups of patients, and Pearson test was adopted to assess the correlation between Hepc content and disease activity.Results: Serum Hepc level of study group was higher than that of control group; serum iron metabolism index SF level was lower than that of control group whereas TF and TIBC levels were higher than those of control group; serum inflammatory factors IL-1α, TNF-α and MCP-1 levels were higher than those of control group whereas IL-4 and IL-10 levels were lower than those of control group; serum immune response indexes IgG and C3 levels were higher than those of control group. The serum Hepc content of patients with RA was directly correlated with the contents of iron metabolism indexes, inflammatory factors and immune response indexes.Conclusion:Hepc level abnormally increases in patients with rheumatoid arthritis combined with anemia, and it is directly correlated with the abnormality of iron metabolism, inflammatory response and immune response. Expression of fluorescent tagged recombinant erythroferrone protein[J].Asian Pacific Journal of Tropical Biomedicine,2018,第7期 Objective: To produce fluorescent tagged recombinant erythroferrone protein(ERFE_eGFP) for laboratory investigations. Methods: Erythroferrone(ERFE) gene was fused to green fluorescent protein(eGFP) gene and cloned in a pSecTag2Hygro plasmid. The constructed plasmid was amplified in Escherichia coli DH5α and the eGFP-fused ERFE(ERFE_eGFP) protein was expressed in human embryonic kidney(HEK293T) cell line. Results: The plasmid constructed from colony C6 contained ERFE_eGFP with the correct restriction sizes of 4.2 kb and expressed secretory ERFE_eGFP fusion protein(approximately size of 75 kDa) in HEK293T cell line. Conclusions: ERFE_eGFP recombinant protein is successfully expressed as a secretory functional protein and could be sensitively detected using fluorometry. This fusion protein might benefit future applications for localization of cellular ERFE receptors and competitive immunoassay of ERFE concentration.

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